Tecnology 3 - A Kit for Parentage Verification in Buffaloes (Bubalus bubalis)
| 1. | Current IP Status: Complete application filed on 22nd December 2011 to Indian Patent Office, New Delhi.
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| 2. | Technology introduction and description: The present technology relates to a kit for determining parentage in buffaloes using distinct microsatellite loci. No parentage verification kit is presently available for buffaloes and the conventional techniques like blood groups, serum proteins and major histo-compatibility antigens have been utilized for solving parentage issues. However, the tests are usually not conclusive due to lack of high degree of polymorphism and are cumbersome to perform. The recent developments have led to use of DNA marker technology which provide very high accuracy. The DNA polymorphism for parentage/paternity testing include restriction fragment length polymorphism, DNA fingerprinting using multi-locus, mini-satellite and oligo-synthetic probes and PCR based amplification of mini satellites and microsatellites. The PCR -RFLP generally have low level of polymorphism and low polymorphic information content while the DNA fingerprints used in probes are difficult to interpret owing to complex nature of banding patterns obtained. No molecular based technology is available till date to answer the correctness of the parentage of the progeny or identity of the daughters in buffaloes. The Kit has been developed for Parentage/ Paternity confirmation using DNA markers in buffaloes. A core set of 15 microsatellite markers each from cattle genome database have been identified which are amplifiable in a single multiplex PCR reaction in buffalo. The markers selected are highly polymorphic, have high heterozygosity, located on different chromosomes and provide a very high probability of exclusion.
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| 3. | Benefits, usefulness, problem solving, business opportunities from the technology to the Client(s):
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| - This is the first kit of DNA based markers for parentage/paternity testing in buffaloes.
- The developed kit utilises a single multiplex PCR reaction-having 15 genetic markers.
- The kit has a very high probability of exclusion.
- The kit has been developed using large buffalo populations from all parts of the country and has performed well in amplification of DNA from vast and varied geographical locations- all markers are polymorphic and exhibit heterozygosity.
- It performs equally well with swamp buffaloes.
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| 4. | Prospective Client(s)/ Investors of the Technology: The clients for the technology are the farmers having buffaloes and wish to carry out the parentage verification.
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| 5. | Expected price of the Technology: Negotiable .
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