Tecnology 12 -
DNA test for mutton quality traits in sheep
| 1. | Technology introduction and description: The growth differentiation factor 8 (GDF8) or Myostatin gene located on sheep (ovine) chromosome 2, is a candidate gene, influencing mutton production. The SNP g.-41C>A identified in the 5’ UTR of GDF8 gene has been associated with increased forequarter weight, mutton tenderness and loin yield, whereas SNP g.6223A>G in the 3’UTR is associated with hyper-muscularity. Two simple, cost effective tetra-primer amplification refractory mutation system polymerase chain reaction (T-ARMS-PCR) assays were designed and optimized to discriminate the alleles at both SNPs. The genotypes were validated by sequencing. The T-ARMS-PCR assays were then used for genotyping 440 samples of five Indian sheep populations. The T-ARMS-PCR assay provides a simple rapid method with increased throughput for allelic discrimination at the GDF8 SNPs in sheep.
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| 2. | Benefits, usefulness, problem solving, business opportunities from the technology to the Client(s): Genetic screening of animals at these two loci will facilitate genetic improvement by selection of animals with desirable traits.
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| 3. | Prospective Client(s)/ Investors of the Technology: Genetic testing laboratories/ Govt. Agencies involved in sheep improvement program
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| 4. | Potential Customers of the Technology: Sheep farmers, Meat Entrepreneurs and Breeding Companies
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